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. 2019 Oct 10;14(10):e0223509. doi: 10.1371/journal.pone.0223509

Fig 3. Isoflurane selectively increased the expression level of apoptotic markers in neonates.

Fig 3

Representative Western blotting images of caspase 9, caspase 12, Bax, and BCL-2 from neonates (A) and old mice (B). Densitometry analysis of the caspase 9 (C), caspase 12 (D), Bax/BCL-2 (E) bands relative to their respective β-actin loading control band in neonates. Densitometry analysis of the caspase 9 (G), caspase 12 (H), Bax/BCL-2 (I) bands relative to their respective β-actin loading control band in the old mice. Statistical significance indicated with asterisks: **p<0.01 and ***p<0.001. N ≥8 animals per group and counts are given as cells/mm2. Scale bar is 100μm.