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. 2019 Oct 10;14(10):e0223096. doi: 10.1371/journal.pone.0223096

Fig 6. Screening of platelet inhibitors with optimized bioluminescence analysis of released ATP.

Fig 6

Washed platelets resuspended in HBSS or Tyrode’s buffer were seeded in white 96-well plate. Subsequently, the platelets were pretreated with Ly294002 or Staurosporine for 5 min and then stimulated with thrombin at 37°C for 20 min. Bioluminescence analyses of ATP released by activated platelets were performed. Inhibition rates and IC50 of Ly294002 (A and B) or Staurosporine (C and D) on ATP release of activated platelets were calculated. Data are representative of three independent experiments.