Table 1. Different UAS-RNAi lines tested and a summary of their phenotypes.
| UAS-RNAi against | Stock ID | tub-Gal4 phenotype | nub-Gal4 phenotype | ap-Gal4 phenotype | Modify Nub-Gal4 > UAS-Hippo-RNAi phenotype? |
|---|---|---|---|---|---|
| Fer1HCH (strong) |
VDRC KK12925 or GD102406 (Overlapping target sequence) |
Lethal before 3rd instar | Pupal lethal | Pupal lethal | 12925 phenotype is dominant, pupal lethal at 25 and 18C |
| Fer1HCH (weak) |
VDRC GD49536 or GD49537 (Identical target sequence) |
Lethal before 3rd instar | Smaller wings | Smaller wings | Yes, mild suppression (5%) |
| Fer1HCH | BL 60000 | Lethal before 3rd instar | Very mild (3% reduction) | Smaller wings | No |
| Fer2LCH | VDRC GD14491 |
pupal lethal | Smaller wings (14%) |
Yes, mild suppression (5%) | |
| Fer2LCH | VDRC KK106960 |
pupal lethal | Very mild (5% reduction) | Yes | |
| Fer2LCH (strongest) |
BL44067 | pupal or earlier lethal | Very mild (4% reduction) | Yes, suppression (10%) | |
| Fer2LCH (weakest) |
BL60354 | semi-lethal, about half can hatch while some die as pupae |