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| Target gene | Primer | Sequence (5’-3’) | Size (bp) | Tm* (°C) | Efficiency (%) | Error |
|---|---|---|---|---|---|---|
|
vanC1 |
VanC1-F | TGCTTGTGATGCGATTTCTC | 204 | 83.57 ± 0.07 | 91.44 ± 1.41 | 0.05 ± 0.03 |
| VanC1-R | ATCGCTCCTTGATGGTGAC | |||||
| vanC2/3 | VanC23-F | GGGAAGATGGCAGTATCCAA | 102 | 80.34 ± 0.09 | 94.81 ± 3.09 | 0.04 ± 0.03 |
| VanC23-R | GCAGCAGCCATTTGTTCATA |
*Melting temperature (Tm), shown as mean ± standard error based on standard curves generated in this study.
