Figure 5.

Loss of Fzd3 and Fzd6 disrupts the polarized distribution of the remaining core PCP proteins. A, A′, B, B′, Immunofluorescent labeling of VANGL2 or CELSR1 (magenta) on the apical surface of the organ of Corti shows the asymmetric distribution of these proteins at hair cell to supporting cell junctions (arrowheads), and between nonsensory cell boundaries (arrows) in the greater epithelial ridge of littermate controls. C, C′, D, D′, In Fzd3;Fzd6 dKOs, VANGL2 and CELSR1 distribution is disrupted around hair cells (examples marked by asterisk) and throughout the GER region (bracket). Moreover, the localization of VANGL2 and CELSR1 appear to be completely lost in the OHC region. E, E′, F, F′, The asymmetric distribution of VANGL2 and CELSR1 at basolateral membrane of supporting cells in control tissue resembles FZD distribution at this location. G, G′, H, H′, In Fzd3;Fzd6 dKOs, VANGL2 and CELSR1 levels appear reduced and the proteins encircle many supporting cells. All images are from E18.5 cochlea. Supporting cell nuclei are identified based upon DAPI staining of nuclei: IPCs, OPCs, first (D1), second (D2), and third (D3) row Deiters' cells. Scale bars, 10 μm.