Figure 8.

WNT mediated signaling establishes PCP axis in cochlear-supporting cells. The asymmetric distribution of VANGL2 and CELSR1 along the basolateral boundaries of cochlear-supporting cells of littermate controls (A–A″) is disrupted in Pax2-Cre;Porcn CKOs (B–B″). Arrowheads indicate examples of asymmetric protein distribution in controls. Asterisks indicate examples of supporting cells that lack asymmetric protein distributions in CKOs. Supporting cell nuclei are identified based upon DAPI staining of nuclei; IPCs, OPCs, first (D1), second (D2), and third (D3) row Deiters' cells. Scale bars, 10 μm.