FIG 2.

Effect of V326N mutation on amyloid formation. Cell surface amyloid formation was monitored with ThT in adhesion assays with BSA-coated magnetic beads (dark spheres, 1-μm diameter). Confocal microscopy was used to examine S. cerevisiae cells containing the empty vector (EV), cells expressing Als1p^WT, and cells expressing Als1p^V326N. Pictures were taken at 1,000× total magnification. Overall, the aggregates were of similar size to those shown in Fig. 1C. For the purposes of fluorescence comparison, we illustrate aggregates of Als1p^WT and Als1p^V326N that are of similar size.