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. 2019 Oct 1;8:e49062. doi: 10.7554/eLife.49062

Video 1. In vitro ovulation of a Ciona ovary.

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DOI: 10.7554/eLife.49062.003

An isolated Ciona ovary was cut in half and incubated in ASW for 16 hr. Time-lapse images were captured every 5 min. Numerous follicles were in turns ovulated from the ovary. The higher-magnification movie of the boxed area indicates that the rupturing of the outer follicular cell layer leads to follicle release.