Video 2. In vitro oocyte maturation and ovulation of a Ciona follicle.

Download video file ^{(1.1MB, mp4)}

DOI: 10.7554/eLife.49062.004

An immature/pre-ovulatory (stage III) follicle was incubated in ASW for 3 hr. Time-lapse images were captured every 20 s. The follicle structure is illustrated at the right. After germinal vesicle breakdown (GVBD) occurred, the outer follicular cell layer was ruptured, and the follicle was released into ASW, reproducing the ovulation observed in the ovary (Video 1).