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. 2019 Oct 1;8:e49062. doi: 10.7554/eLife.49062

Video 4. The MEK inhibitor U0126 inhibits Ciona GVBD and ovulation.

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DOI: 10.7554/eLife.49062.014

Isolated early stage III follicles were incubated with (right) or without (left) 10 μM U0126 for 24 hr. Time-lapse images were captured every 5 min. GVBD and ovulation were observed in the control follicle, whereas those were inhibited in the U0126-treated follicle.