Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Ciona intestinalis type A) | CiVp | Ciona Ghost Database (http://ghost.zool.kyoto-u.ac.jp/cgi-bin/gb2/gbrowse/kh/) | KH.C6.11. | |
| Gene (Ciona intestinalis type A) | CiVpr | Ciona Ghost Database (http://ghost.zool.kyoto-u.ac.jp/cgi-bin/gb2/gbrowse/kh/) | KH.C9.885. | |
| Gene (Ciona intestinalis type A) | CiErk1/2 | Ciona Ghost Database (http://ghost.zool.kyoto-u.ac.jp/cgi-bin/gb2/gbrowse/kh/) | KH.L153.20. | |
| Gene (Ciona intestinalis type A) | CiCcnb | Ciona Ghost Database (http://ghost.zool.kyoto-u.ac.jp/cgi-bin/gb2/gbrowse/kh/) | KH.C4.213. | |
| Gene (Ciona intestinalis type A) | CiCdk1 | Ciona Ghost Database (http://ghost.zool.kyoto-u.ac.jp/cgi-bin/gb2/gbrowse/kh/) | KH.C12.372. | |
| Gene (Ciona intestinalis type A) | CiMmp2/9/13 | Ciona Ghost Database (http://ghost.zool.kyoto-u.ac.jp/cgi-bin/gb2/gbrowse/kh/) | KH.L76.4. | |
| Recombinant DNA reagent | pET21a-CiMMP2/9/13 (plasmid) | This paper | Expression vector of Ci-MMP2/9/13 | |
| Strain, strain background (Escherichia coli) | Rossetta 2 (DE3) | Novagen | Cat.#:71400–3 | Competent cells for rCi-MMP2/9/13 expression |
| Antibody | anti-ERK (rabbit polyclonal) |
Cell Signaling Technology | Cat.#:9102S; RRID:AB_330744 |
WB (1:500) |
| Antibody | anti-pERK (rabbit polyclonal) |
Cell Signaling Technology | Cat.#:9101S; RRID:AB_331646 |
IF (1:200); WB (1:1000) |
| Antibody | anti-PSTAIR (mouse monoclonal) |
Abcam | Cat.#:Ab10345; RRID:AB_297080 |
WB (1:1000) |
| Antibody | anti-CiMMP2/9/13 | This paper | IHC (1:400); WB (1:3000) | |
| Commercial assay or kit | MESACUP Cdc2/Cdk1 Kinase Assay kit | MBL | Cat.#:5235 | |
| Commercial assay or kit | Universal Elite ABC kit | Vector Laboratories | Cat.#:PK-7200 | |
| Peptide, recombinant protein | CiVP | Kawada et al., 2008 (PMID: 18586058) | 5 μM | |
| Chemical compound, drug | U0126 | Promega | Cat.#:V1121 | 10 μM |
| Chemical compound, drug | Ro-3306 | Abcam | Cat.#:ab141491 | 1 μM |
| Chemical compound, drug | MMP-2/MMP-9 inhibitor II | Calbiochem | Cat.#:444249 | 20 μM |
| Chemical compound, drug | DQ Collagen, type I | Thermo Fisher Scientific | Cat.#:D12060 | 50 μg/ml |
| Chemical compound, drug | DQ Collagen, type IV | Thermo Fisher Scientific | Cat.#:D12052 | 50 μg/ml |
| Software, algorithm | Free-JSTAT statistical software | FreeJSTAT (http://toukeijstat.web.fc2.com/) | Version 22.0E | |
| Software, algorithm | Fiji software | Fiji (http://fiji.sc/); Schindelin et al., 2012 (PMID: 22743772) |
RRID:SCR_002285 | |
| Sequence-based reagent | Primers | This paper | Sequences are listed in Table 3 |