Fig. 4. Deletion of HSP60 reduced mitochondrial membrane potential in yolk sac erythrocytes.

Single cells were prepared from control and HSP60^CKO yolk sacs at E8.5, and flow cytometry was performed to measure mitochondrial membrane potential using tetramethylrhodamine (TMRM) in Ter119 positive (Ter119⁺) and Ter119 negative (Ter119⁻) cells. a Distribution of TMRM fluorescence of Ter119⁻ and Ter119⁺ cells in control and HSP60^CKO yolk sac cells. b Statistical analysis showing reduced TMRM fluorescence in HSP60^CKO Ter119⁺ cells. n = 4 mice per group. All data represent mean ± SEM. Significance was determined using a 2-tailed, unpaired Student’s t test. *p < 0.05 versus control