Fig. 5. HSP60 deletion affected VDAC expression and Cyt C release in yolk sac erythrocytes.

a Immunofluorescence staining of GATA1 and VDAC in control and HSP60^CKO yolk sacs at E9.0. HSP60 deletion abolished VDAC expression in yolk sac erythrocytes. Data are representative of at least three independent experiments. Scale bar, 20 μm. b Immunofluorescence staining of GATA1 and Cyt C in control and mutant yolk sacs at E9.0. The GATA1 positive cells with released Cyt C were characterized with no perinuclear immunofluorescence (white arrows). Data are representative of at least three independent experiments. Scale bar, 20 μm. c Statistical analysis showing increased numbers of the cells with released Cyt C in mutant erythrocytes. n = 13 from 5 control yolk sacs and 9 from 3 HSP60^CKO yolk sacs. All data represent mean ± SEM. Statistical significance was determined by Mann-Whitney U test. *p < 0.001 versus control