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. 2019 Oct 10;9:14511. doi: 10.1038/s41598-019-50962-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miRNA differential accumulation (DA) during callus induction from maize embryos collected at different developmental stages. (a) DA of miRNAs between zygotic embryos developmental stages (IE15, IE23 and ME). Comparisons were done for IE15 vs. IE23, IE15 vs. ME and IE23 vs. ME. (b) DA of miRNAs during the induction of Somatic Embryogenesis. Comparisons were done for IE15 vs. C15, IE23 vs. C23 and ME vs. CM. (c) DA of miRNAs in callus tissues obtained from developmentally distinct embryos at one month of induction. Comparisons were done for C15 vs. C23, C15 vs. CM and C23 vs. CM. Values of DA were expressed as the log2 Fold Change (log2FC) and significant values indicated as follows: *p < 0.05; **p < 0.01; ***p < 0.001.