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. 2019 Oct 10;10(10):768. doi: 10.1038/s41419-019-2000-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a RT-qPCR analysis for the indicated genes in HNF4α-silenced HepE14 (HepE14 siHNF4) and colon SW620 cells (SW620 siHNF4), compared with GFP-silenced control cells. The values are calculated by the 2^(−ΔCt) method, expressed as fold of expression versus the control (arbitrary value = 1) and shown as means ± S.E.M. of at least three independent experiments. Statistically significant differences are reported (*p < 0.05; **p < 0.01). b Western blotting analysis for YAP and HNF4α in HNF4α-silenced HepE14 and SW620 cells (siHNF4), compared with GFP-silenced control cells (siCTR). GAPDH was used as a loading control. c RT-qPCR analysis of YAP and HNF4α in liver samples from four hepatocyte-specific HNF4α knockout (KO) mice⁴⁸ and four wild-type (WT) mice. Statistically significant differences between the two groups of mice are reported (*p < 0.05). d qPCR analysis of ChIP assays with α-HNF4α antibody (HNF4), and as control, with normal rabbit IgG on chromatin from HepE14 cells. Values derived from at least four independent experiments are calculated as IP/IgG and reported as means ± S.E.M. respect to the IgG sample (arbitrary value = 1). RPL30 promoter was used as negative control (***p < 0.001; ns = not significant). e qPCR analysis of ChIP assays with α-H3K27me3 antibody (H3K27me3), and as control, with normal rabbit IgG on the chromatin from HepE14 cells. Values derived from at least three independent experiments are calculated as IP/IgG and reported as means ± S.E.M. respect to the IgG sample (arbitrary value = 1). RPL30 and Neurogenin promoters were used as negative and positive control, respectively (*p < 0.05; ns = not significant)