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. 2019 Oct 10;9:14616. doi: 10.1038/s41598-019-51144-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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JPH 203 induced cell cycle arrest in human ATC cells. (A) Cell cycle analysis after 24 hours of JPH203 treatment. The percentages of cell populations of different cell cycle phases are shown in the upper-right corner. After JPH203 treatments, 8505C, OCUT-2, and OCUT-6 were blocked in the G1/G0 phase, and there was reduction in the S and G2/M phases. (B) JPH203 treatment affected the distribution of cell cycle. Different cell cycle phases were quantified by DAPI staining, followed by fluorescence-activated cell sorting analysis in our human ATC cells. (C) JPH203 treatment lengthened the G1/G0 phase in our human ATC cells (**p < 0.01, respectively). (D) A representative western blot for expression of cell cycle-associated proteins, including cyclin D1, CDK4 and E2F1 in 8505C, OCUT-2, and OCUT-6 cells after treatment with 100 µM JPH203 for 24 h or control DMSO. (E–G) The band intensities were quantified, and compared after normalize to α-tubulin (*p < 0.05, **p < 0.01, ***p < 0.001).