Table 1.

The results of B6 allele-targeting mutation introduction by WT-Cas9 or HypaCas9

Cross	Injected RNA	Embryos injected*	Mutated		Nonmutated (%)
			Biallelic (%)	Monoallelic (B6-specific) (%)
♀B6 × ♂B6	WT-Cas9 + gRNA1	19	15 (78.9)	0 (0)	4 (21.1)
♀B6 × ♂D2	WT-Cas9 + gRNA1	22	0 (0)	9 (40.9)	13 (59.1)
♀B6 × ♂D2	WT-Cas9 + gRNA2	21	21 (100)	0 (0)	0 (0)
♀B6 × ♂D2	HypaCas9 + gRNA2	23	0 (0)	23 (100)	0 (0)

^*The total number of embryos in two replicate experiments is shown