Table 2.
Summary of conditions and sample sizes for each assay reported.
| Assay | Species | Culture date | Assay type | Embryo/larval age | Conc. agarose (%) | Vol. agarose (ml) | Vol. FSW over agarose (ml) | Embryo/larval embedding technique | No. Reps | No. embryos/larvae per replicate (mean ± SD) | Embedded duration (days) | No. days read post settlement induction |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Embryogenesis | Montipora digitata | 6/04/2018 | 6-well plate | 2 hr | 0.65 | 5.0 | 5.0 | embryo first | 6 | 10 ± 0 | 3 | NA |
| Montipora digitata | 4/04/2108 | Petri dish | 2 hr | 1.00 | 30.0 | 20.0 | embryo first | 1 | 30 | 2 | NA | |
| Montipora digitata | 4/04/2018 | 6-well plate | 20 hr | 0.80 | 3.0 | 7.0 | embryo first | 9 | 10 ± 2 | 4 | NA | |
| Montipora digitata | 4/04/2018 | 6-well plate | 24 hr | 0.80 | 3.0 | 7.0 | embryo first | 6 | 5 ± 0 | 4 | NA | |
| Montipora digitata | 4/04/2018 & 6/04/2018 | 48-well plate | 96 hr & 144 hr | 0.80 | 0.8 | 0.8 | embryo first | 29 | 1 | 3 | NA | |
| Short-term survival | Montipora digitata | 4/04/2018 | 6-well plate | 1 day | 0.65 | 3.0 | 7.0 | embryos first | 6 | 5 ± 0 | 4 | NA |
| Acropora millepora | 28/10/2018 | 6-well plate | 13 days | 0.8 | 7.0 | 0.0 | agar first | 6 | 11 ± 2 | 4 | NA | |
| Favia matthaii | 28/11/2018 | 6-well plate | 36 days | 0.8 | 7.0 | 0.0 | agar first | 6 | 15 ± 1 | 5 | NA | |
| Mycedium elephantotus | 28/11/2018 | 6-well plate | 36 days | 0.8 | 7.0 | 0.0 | agar first | 6 | 17 ± 2 | 6 | NA | |
| Platygyra daedalea | 29/10/2018 | 6-well plate | 12 days | 0.8 | 7.0 | 0.0 | agar first | 6 | 9 ± 4 | 4 | NA | |
| Long-term survival | Montipora digitata | 4/04/2018 & 6/04/2018 | 48-well plate | 96 hr & 144 hr | 0.8 | 0.8 | 0.8 | embryo first | 29 | 1 | cont. | NA |
| Settlement | Montipora digitata | 4/04/2018 | 6-well plate | 17 days | 0.8 | 7.0 | 0.0 | agar first | 6 | 8 ± 1 | 1 | 2 |
| Acropora millepora | 28/10/2018 | 6-well plate | 17 days | 0.8 | 7.0 | 0.0 | agar first | 6 | 10 ± 1 | 4 | 1 | |
| Favia matthaii | 28/11/2018 | 6-well plate | 41 days | 0.8 | 7.0 | 0.0 | agar first | 6 | 11 ± 2 | 5 | 2 | |
| Mycedium elephantotus | 28/11/2018 | 6-well plate | 42 days | 0.8 | 7.0 | 0.0 | agar first | 6 | 15 ± 1 | 6 | 2 | |
| Platygyra daedalea | 29/10/2018 | 6-well plate | 16 days | 0.8 | 7.0 | 0.0 | agar first | 6 | 10 ± 1 | 4 | 1 | |
| Spat | Acropora millepora | 9/11/2017 | Petri dish | 14 days post settlement | 0.7 | 5.0 | 5.0 | spat first | 1 | 5 | 7 | NA |
We note that the volume of agarose (ml) equalled the volume of the filtered seawater (FSW) equivalent in negative controls.