Fig. 5.

Electrophysiological measurements of unitary TbMscL channels. a Representative patch clamp recordings at 20 mV from patches excised from GUVs containing WT, L89W, and L89R1 (±5 mM DTT) TbMscL protein. The pressure applied during the recording is indicated below each trace. The current levels representing the closed (C) and fully opened (O, ~60  pA for WT, L89W and L89R1, and 50 pA for L89R1 plus DTT) channels are indicated. Note that channels opening to a subconductance level (S, ~5 pA) were also recorded with L89W and L89R1 (modified channel). b Distribution of threshold pressures, applied at 10 mmHg intervals, at which pressure-activated MscL full channel openings were first observed. Data points represent individual excised patches, whiskers show the full range of values, the boxes represent the 25–75 percentile range, and the horizontal line is the median. The p values from independent samples Kruskal–Wallis Test with pairwise comparisons are indicated where these were <0.05, with respect to WT channels