Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 10;9:14490. doi: 10.1038/s41598-019-50899-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Enrichment of hESC-CMs by mBax_499 mRNA and modelling to predict yield. (a) Representative confocal images of hESC-CMs that were either untreated or treated with mBax_499 mRNA for 24 h and stained for F-actin filaments by phalloidin (red), counterstained with NucBlue (nucleus, blue) and sarcomeric α-actinin (green). Scale bar: 100 µm. (b) Quantification of the percentage of actinin fiber alignment along the major strain axis in randomly cropped confocal images of treated hESC-CMs (n = 27) and untreated hESC-CMs (n = 29) using the Image J software and the OrientationJ Distribution plugin. (c) Co-staining of cells with SIRPa-APC and Annexin V-FITC before treatment (left) and after 24 h treatment with mBax_499 mRNA (right). (d) Experimental results vs. model for outcome prediction of % hESC-CMs after treatment. (e) FACS analysis of cells stained with cTNT after three consecutive treatments with mBax_499 mRNA. (f) Utilizing the model for repetition of treatment prediction (one through four treatments) and comparison to experimental results of one, two and three repeated treatments with mBax_499 mRNA. Data are represented as mean (SD). p value was generated using two-tailed unpaired student’s t-test. *p < 0.05.