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. 2019 Oct 10;9:14598. doi: 10.1038/s41598-019-50775-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genotype-dependent differences in the auditory brainstem response (ABR) amplitude-intensity profiles of PACAP-deficient (KO) and wild-type (WT) mice at 16.4, 32.8 and 65.6 kHz. (a) Asterisks indicate significant differences between KO and WT mice. Mean ± SEM; 2-way-ANOVA, Bonferroni post-hoc test. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05 vs. WT values at the corresponding stimulus frequency and intensity. (b) Example for absolute amplitude measurement at peak II. (c) Average traces (±SEM) at 32.8 kHz, 90 dB demonstrate the waveform differences between WT and KO mice. Two-headed arrows (↕) demonstrate the difference between peak I amplitude values (black: WT, red: KO).