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. 2019 Oct 10;9:14493. doi: 10.1038/s41598-019-50769-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RCFE treatment led to inhibition of STAT3 phosphorylation. Western blot analysis of MCF-7 (A) and MDA-MB-231 (B) cell lysates with phosphor-STAT3 (Tyr705) and STAT3 antibodies. The quantitation of the expression was presented in right panels. Doxorubicin (Doxo) was used as positive control. Statistical differences of three independent experiments were analyzed with one-way ANOVA test. p value < 0.05 was considered significant. (C) Schematic representation of the metastasis inhibition and apoptosis induction by RCFE for its anti-cancer activity.