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. 2019 Oct 10;9:14647. doi: 10.1038/s41598-019-50690-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Force inference at the single junction scale in the Drosophila notum. (A) Subregion of the Drosophila notum 21 h after pupa formation. Scissors show ablation spots where recoil velocities will be measured. Insets show post-ablation snapshots of the considered junctions. Scale bar: 5 μm. (B) Inferred tension map of the tissue region in (A) before the ablations. Red arrows indicate the location of ablations, where inferred tensions are extracted and compared to experimental recoil velocities. (C) Opening dynamics and initial recoil velocity. The red line shows a linear fit of the first 5 seconds, which is used to determine the initial recoil velocity. (D) Inferred tension vs. opening velocity (N = 31 laser cuts from 10 pupae). Pearson’s correlation coefficient is 0.59. Spearman’s correlation coefficient is 0.63.