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. 2019 Oct 10;9:14582. doi: 10.1038/s41598-019-51136-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sparse Partial‐least‐squares Discriminant Analysis plots of T1 group (a) and T2 group (b) after Amplicon Sequences Variants selection (139 and 151, respectively). The feed factor standard protein (SP) vs low protein (LP) in T1 and carotene-enriched (CE) vs control diet (NC) in T2 factors were used to distinguish groups. At time-point T1, purple color represents the low-protein diet and pink color the standard-protein diet. At time-point T2, purple color represents the carotene-enriched diet and pink color the control diet.