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. 2019 Oct 10;10(10):775. doi: 10.1038/s41419-019-2005-3

Fig. 3. poFUT1 is involved in hESCs motility and angiogenesis and vascular remodeling ability.

Fig. 3

hESCs transfected with scramble-siRNA, poFUT-siRNA, or co-transfected with poFUT1-siRNA and poFUT1-cDNA. a–d Real-time PCR (a, c) and western blot (b, d) analyses of poFUT1, CD31, and VE-cadherin at the relative transcript level and protein level. e Immunofluorescent staining of poFUT1, CD31, and VE-cadherin in hESCs. f F-actin cytoskeleton change of hESCs. g Wound-healing assay detected hESC motility. h Tube-formation assay analyzes hESC angiogenesis ability. i Tube-formation assay analyzes coangiogenesis of hESCs and HUVECs. Significance was indicated by *P < 0.05, **P < 0.01, ***P < 0.001