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. 2019 Oct 10;10(10):775. doi: 10.1038/s41419-019-2005-3

Fig. 4. poFUT1 increase the biosynthesis of O-fucose on uPA and activated Rho signaling pathway.

Fig. 4

a O-fucose detection assay analysis of O-fucosylation on hESCs after transfection with scramble-siRNA, poFUT-siRNA, or co-transfection with poFUT1-siRNA and poFUT1-cDNA. b Immunoprecipitation and western blot analysis of O-fucosylation on uPA in hESCs after transfected with scramble-siRNA, poFUT-siRNA, or co-transfected with poFUT1-siRNA and poFUT1-cDNA. Immunoprecipitation (IP): anti-uPA antibody pulled down protein. Immunoblot (IB): detection of O-fucosylation by O-fucos detection assay. c IP and western blot analysis of O-fucosylation on uPA in hESCs transfected with uPA-cDNA or uPA-M-cDNA. uPA-M-cDNA: uPA mutation cDNA. e Western blot and statistical analysis of p-LIMK, LIMK, Rho-GTP, RhoA, p-Confilin, Confilin in hESCs transfected with uPA-cDNA or uPA-M-cDNA. f Western blot and statistical analysis activation of RhoA signaling pathway in hESCs transfected with poFUT1-cDNA or with uPA antibody, RhoA inhibitor blocking. Significance was indicated by *P < 0.05, **P < 0.01, ***P < 0.001