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. 2019 Oct 10;9:14664. doi: 10.1038/s41598-019-51140-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blotting analysis of p-GSK3β and GSK-3β in rat spinal dorsal horns after 7 days and 14 days of CCI. The levels of pGSK-3β (Ser9) were clearly reduced in the left spinal cord of CCI rats at 7 days (A) and 14 (B) days after surgery when compared with the sham group (A: p < 0.05, n = 5, t = 5.2, df = 8; B: p < 0.05, n = 5, t = 3.788, df = 8). However, ghrelin treatment significantly counteracted this change (A: p < 0.05, n = 5, t = 5.981, df = 8; B: p < 0.05, n = 5, t = 2.867, df = 8). These semiquantitative measurements are expressed as ratios of pGSK-3β to GSK-3β. Results are presented as mean ± SD. n = 5. ^##p < 0.01 vs Sham group; ^$p < 0.05 vs CCI group; ^$$p < 0.01 vs CCI group.