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. 2019 Oct 10;9:14664. doi: 10.1038/s41598-019-51140-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical analysis of pGSK3β in rat spinal dorsal horns after 7 days of CCI. To investigate the levels of pGSK-3β in the spinal dorsal horns of CCI rats, we also immunostained spinal cord sections with anti-pGSK-3β antibodies at day 7 after CCI. Cytosolic pGSK-3β was detected in the spinal dorsal horns of all groups. The levels of pGSK-3β in the CCI group were significantly decreased compared with those in the sham group (p < 0.05, n = 5, t = 9.701, df = 8). Compared with the CCI group, the levels of pGSK-3β were significantly increased in the ghrelin-treated groups (p < 0.05, n = 5, t = 6.519, df = 8). Results are presented as mean ± SD. n = 5. **p < 0.01 vs Normal group; ^##p < 0.01 vs Sham group; ^$$p < 0.01 vs CCI group.