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. 2019 Oct 10;9:14664. doi: 10.1038/s41598-019-51140-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blotting analysis of Wnt3a protein expression in rat spinal dorsal horns after 7 days and 14 days of CCI. To confirm the change in the Wnt/β-catenin signaling pathway after CCI and intrathecal injection of ghrelin, we investigated the protein expression levels of Wnt3a in rat spinal dorsal horn tissues by western blotting analysis. Both 7 days (A) and 14 days (B) after CCI, the expression levels of Wnt3a were significantly increased (A: p < 0.05, n = 5, t = 6.471, df = 8; B: p < 0.05, n = 5, t = 6.675, df = 8). After continuing the intrathecal injection of ghrelin for 7 days, the expression levels of Wnt3a were not reduced when compared with the CCI group (A: p > 0.05, n = 5, t = 0.222, df = 8; B: p > 0.05, n = 5, t = 0.986, df = 8). These semiquantitative measurements were expressed as the ratios of Wnt3a to GAPDH. Results are presented as mean ± SD. n = 5. ^##p < 0.01 vs Sham group.