Table 2.
Primers used in this study.
| Name | Sequence 5′-3′ |
|---|---|
| GFP-PacI-F | AATGTTAATTAAGAAGGAGATATACATCATGTCGAAGGGCGAGGAGCTGTTC |
| GFP-linker-R | GAGCCGCCGCCCGAGCCGCCGCCGCCCGAGCCGCCCTTGTACAGCTCGTCCATGCC |
| mRFP-linker-F | GCGGCGGCTCGGGCGGCGGCTCGTCGGGCGGCGCCTCCTCCGAGGACGTCATCAAG |
| mRFP-EcoRV-R | AATCTTGATATCTTAGCGCCGGTGGAGTGGCGG |
| GFP-D103N-Mut-F | CCTTCAAGGACAACGGTAACTACAAGAC |
| GFP-D103N-Mut-R | GTCTTGTAGTTACCGTTGTCCTTGAAGG |
| BamHI-HspX-F | AACACGGATCCAATGACCAAACTTCCTGAAC |
| HspX-GFP-R | GGTGGCCACCGGCGGGTCCGGGCTGACGGTCTCCACCGCG |
| HspX-GFP-F | GACCCGCCGGTGGCCACCATGTCGAAGGGCGAGGAGC |
| HindIII-GFP-R | TTCGCTAAGCTTCTACTTGTACAGCTCGTCC |
| HspXKO-1 | ACCGTGCGGCACGGGGAGAT |
| HspXKO-2 | GGCCTCTCGAAGCGGTCCTCCTCACTCGTAGG |
| HspXKO-3 | AGGAGGACCGCTTCGAGAGGCCTATAACTTCG |
| HspXKO-4 | CGGCCATGGCGAAGTACTTCTAGACTCGAGATAACTTCG |
| HspXKO-5 | TCTAGAAGTACTTCGCCATGGCCGTGAACA |
| HspXKO-6 | GCCCGGTGCCCATGGAC |
| RBS-HspX-GFP-F | AAGGAGATATACATCATGACCAAACTTCCTGAACGATCACGAG |
| pUVtet-△N35HspX-GFPa | ATAGGCTCTGGGAGTACCCG GACCACATCATCCGGATCGAGG |
| pUVtet-△N35HspX-GFPb | GAAAAGTTCTTCTCCTTTACT CGGGCTGACGGTCTCCACCGCG |
| pUVtet-△N35HspX-GFPc | CGGTGGAGACCGTCAGCCCG AGTAAAGGAGAAGAACTTTTCAC |
| pUVtet-△N35HspX-GFPd | CCCCAATTAATTAGCTAAA TCAACATTTGTATAGTTCATC |
| pUVtet-HspX-GFPe | CGGGTACTCCCAGAGCCTATCTATC |
| pUVtet-HspX-GFPf | TTTAGCTAATTAATTGGGGACCC |
| pUVtet-△C HspX-GFPa | ATAGGCTCTGGGAGTACCCG ATGACCAAACTTCCTGAACGATC |
| pUVtet-△C HspX-GFPb | GAAAAGTTCTTCTCCTTTACT GAACCCGAGGCAGCCGCCGAGAAG |
| pUVtet-△C HspX-GFPc | CCCGAGGCAGCCGCCGAGAA AGTAAAGGAGAAGAACTTTTCAC |
| pUVtet-△C HspX-GFPd | CCCCAATTAATTAGCTAAA TCAACATTTGTATAGTTCATC |
| pUVtet-△N△CHspX-GFPa | ATAGGCTCTGGGAGTACCCG GACCACATCATCCGGATCGAGG |
| pUVtet-△N△CHspX-GFPb | GAAAAGTTCTTCTCCTTTACT GAACCCGAGGCAGCCGCCGAGAAG |
| pUVtet-△N△CHspX-GFPc | CCCGAGGCAGCCGCCGAGAA AGTAAAGGAGAAGAACTTTTCAC |
| pUVtet-△N△CHspX-GFPd | CCCCAATTAATTAGCTAAA TCAACATTTGTATAGTTCATC |
| mcherry-wtHspX-R | GACGTCCTCGGAGGAGGCCGGGCTGACGGTCTCCACCGCGACA |
| wtHspX-mcherry-F | GTGGAGACCGTCAGCCCGGCCTCCTCCGAGGACGTCATCAAGG |
| HindIII-mcherry-R | CCCAAGCTT TTA ACTGGATCCGCTAGATCCCTGGGAGCC |
| mcherry-N35HspX-R | GAC GTC CTC GGA GGA GGC ACC GAA AAC CGG GCG GAT CGA GGC C |
| N35HspX-mcherry-F | ATC CGC CCG GTT TTC GGT GCC TCC TCC GAG GAC GTC ATC AAG G |
| PacI-RBS-△NHspX-F | GCC TTA ATT AAG AAG GAG ATA TAC ATC GAC CAC ATC ATC CGG ATC GAG GAC GA |
| PacI-SepF-F | CCTTAATTAACAGAAAGGAGGTTAATAATGAGCACACTGCATAAGGTCAAGGCC |
| SepF-linker-HspX-R | TTGGTGCCGCCCGACGAGCCGCCACGGTAGGAGTAGAAGCCCGCCTCGG |
| Linker-HspX-F | GGCGGCTCGTCGGGCGGCACCAAACTTCCTGAACGATCACGAGCACGC |
| HspX-HindIII-R | CCCAAGCTTTTACGGGCTGACGGTCTCCA |
| GLR103-SepF-F | ACCGGCGCCTAAGATCAGAAAGGAGGTTAATAATGAGCAC |
| HspX-Ptet R | GGTCCCCAATTAATTAGCTAAAGCTTGATTTACGGGCTGACGGTCTC |
| mApple-F | CTA GCT AGC AGC AAG GGC GAG GAG AAT AAC ATG GCC ATC ATC AAG GAG TTC ATG CGC |
| mApple-R | CCC AAG CTT TTA GGT CGA GTC CAG GCC CAG CAG CGG GTT CGG GAT CGG CTT GCC CTT GTA CAG |
| Msmeg_6339-F | CCTTAATTAAGAAGGAGATATACATCatggagtcgaacccaccgagtgccgtcgtcga |
| Msmeg_6339-R | CTAGCTAGCgggcgtgacgatgctgaagttcgggt |
| murA-F | CCTTAATTAAGAAGGAGATATACATCgtgagcgagcgtttcgtggtgaccggtggcaa |
| murA-R | CTAGCTAGCcgagcttactctctcgatctcggctc |
| tuf-F | CCTTAATTAAGAAGGAGATATACATCgtggcgaaggcgaagttcgagcggacgaagcc |
| tuf-R | CTAGCTAGCcttgatgatcttggtgacgcggccgg |