FIGURE 8.

GluN2B transmission and NMDAR plasticity in adult mice with a history of AIE following a restraint stress challenge. (A) Time course of NMDAR-EPSC peak amplitudes following a 10-min application of Ro 25–6981. (B) Quantification of the inhibition of NMDAR currents produced by Ro 25–6981, determined by calculating the percentage change in EPSC amplitude from 5 min before Ro addition to 5 min of the peak drug effect (last 5 min of washout). (C) Representative traces of NMDAR-EPSCs recorded from Air-Str (top) and AIE-Str (bottom) mice before (gray and black traces, respectively) and after (red traces) application of Ro 25–6981; vertical scale bar = 100 pA, horizontal scale bar = 200 ms. (D) Plot of the summation index across stimulation frequencies and treatments demonstrating that stress during adulthood was sufficient to restore the sensitization of NMDAR-EPSCs in adults with a history of AIE. A significant effect of treatment was determined by a two-way ANOVA, ^∗∗∗p < 0.001. (E) Representative traces (Air-Str gray, AIE-Str black) demonstrating the ability of acute stress to enhance the temporal summation of NMDAR-EPSCs in adult males with a history of AIE; vertical scale bar = 200 pA, horizontal scale bar = 200 ms. (F) Plot of the summation index across stimulation frequencies find no significant effect of stress in adult female mice with and without a history of AIE.