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Synthesis of trifunctional CLR-epitope-TLR conjugates. Reagents and conditions: (a) Fmoc-SPPS; (b) TFA, DCM; (c) Fmoc-SPPS; (d) 23 (Gential et al., 2019), HCTU, DIPEA, DMF; (e) TFA, TIS, H2O, octanethiol, phenol; (f) CuI, THPTA, DIPEA, DMSO, H2O, (+ NaAsc, Arg, H2O), CLR clusters (19–22).
