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. 2019 Oct 10;10(10):771. doi: 10.1038/s41419-019-2011-5

Fig. 2. LC3 and p62 bait constructs behave normally.

Fig. 2

a Scheme depicting the fusion constructs of LC3 and p62 baits. b Cell lines stably expressing SBP–GFP–LC3 or p62–SBP–GFP were treated with rapamycin (Rapa) alone (1 µM, 6 h) or with rapamycin and bafilomycin A1 (BafA1; 500 nM, 2 h). Scale bar equals 10 µm. c Quantification of GFP containing puncta per cell from images in (b) by high throughput microscopy. d Quantification of relative fluorescence intensity (FI) of the p62–SBP–GFP transgene in response to treatment with rapamycin alone (1 µM, 6 h) or with rapamycin and bafilomycin A1 (500 nM, 2 h). Bars indicate means ± standard deviation of at least three replicates (*p < 0.05, **p < 0.01, and ***p < 0.001, two-tailed Student’s t test, compared to control cells)