Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 10;10:4602. doi: 10.1038/s41467-019-12373-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Improvement of re-vascularization of rat islets and diabetes correction in SCID-Beige mice. a Schematics and a microscopic image of rat islets (green) in a Mesh device. Microvascular mesh is red and nylon grid is blue. b Non-fasting blood glucose (BG) concentration of the mice after transplantation. Grafts were retrieved after different time points. Most of the mice were kept alive for 1 week after retrieval while 3 mice from the Mesh group were used for perfusion studies prior to retrievals. During 42 days of transplantation (n = 9 in No cell, n = 11 in Random, and n = 14 in Mesh): *P < 0.05 and NS (P > 0.05) no significant difference. ANCOVA, time was treated as continuous covariate. c BG concentrations during intraperitoneal glucose tolerance tests (IPGTT) after 30 and 90 days of transplantation. IPGTT at Day 30 (n = 6 in Normal mice and No cell, n = 8 in Random and Mesh). Data are mean ± SEM. *P < 0.05 and NS (P > 0.05) no significant difference. ANCOVA, time was treated as continuous covariate. d Hematoxylin/eosin staining of rat islets and blood vessels in retrieved devices (Day 42) and the number of blood vessels around a rat islet (within a 200 μm distance). Yellow arrowheads point to blood vessels with erythrocytes inside. White arrows point to rat islets. The round material in the cross-sections is nylon grid. No cell group consists of 11 islets pooled from 3 mice; Random group consists of 13 islets pooled from 3 mice; Mesh group consists of 12 islets pooled from 3 mice. *P < 0.05, ***P < 0.001, and NS (P > 0.05) no significant difference. One-way analysis of variance. e Cross-sectional immunostaining images of rat insulin (red) and mouse blood vessels (CD31, green) in a retrieved Mesh device. f Immunostaining images (parallel section) using human (red) and mouse (green) CD31 antibodies indicate the anastomoses between human and mouse blood vessels. g Confocal image of perfused blood vessels in re-vascularized rat islets in the Mesh group after 42 days of transplantation. h Fluorescent images of re-vascularized rat islets after 91 and 112 days of transplantation from the Mesh group. The rat islets expressing GFP are green and perfused blood vessels are red