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. 2019 Oct 10;10(10):770. doi: 10.1038/s41419-019-2013-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Representative images of capillary-like structures. HUVECs were seeded on Matrigel in presence of regular media (RM) or A2780 and SKOV3 cell derived CM or matrine-treated A2780 and SKOV3 cell derived CM. RM served as control set. Scale bar: 20 μm. b The error-bar graphs showed the mean numbers of capillary-like structures formed by HUVECs in different experimental conditions (*P < 0.05, **P < 0.01). c The levels of VEGFA in the media of A2780 and SKOV3 cells treated with matrine were measured by ELISA. Data are represented as means ± SD of three independent experiments (*P < 0.05). d Effect of matrine on the mRNA levels of VEGFA in A2780 and SKOV3 cells. e The protein expressions of VEGFA were significantly decreased in A2780 and SKOV3 cells with the addition of matrine. f Effect of matrine on the mRNA levels of ANG-1 in HUVECs co-cultured with A2780 or SKOV3 cells. g The protein expressions of ANG-1 were decreased by matrine in HUVECs co-cultured with A2780 or SKOV3 cells. h The phosphorylation levels of VEGFR2 and Tie2 were down-regulated by matrine in HUVECs co-cultured with A2780 or SKOV3 cells. GAPDH was used as a loading control