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. 2019 Oct 10;10(10):763. doi: 10.1038/s41419-019-1967-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The normal process of differentiation and the phases of the protocol used in this study. b Representative morphology of cells at different stages observed by phase contrast microscopy. c Summary of the time course and the additions of small molecules for each phase of differentiation. d Copy-number variation (CNV) analysis of hepatocytes from human embryonic stem cells. Scale bars, 200 µm.