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. 2019 Oct 10;9:14661. doi: 10.1038/s41598-019-51092-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Milk EVs restore intestinal permeability in vivo. (A) Quantification of dextran-FITC in the plasma of healthy and colitic mice different times after feeding with one of the three feeding solutions. Fluorescence is expressed as fold change versus control. (B) Dextran-FITC in plasma at day 7. Statistical comparison. For all conditions, statistical significance was determined by one-way Anova with Bonferroni’s post-hoc correction for multiple comparison with p < 0:05 considered significant (n = 10/group). Significance display. *p < 0.05; **p < 0.01; ***p < 0.001. Abbreviations. CS, control solution; DSS, dextran sodium sulfate; EVs, extracellular vesicles; FITC, fluorescein isothiocyanate; P100K, pellet 100,000 g; P35K, pellet 35,000 g.