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. 2019 Oct 10;10(10):764. doi: 10.1038/s41419-019-1996-0

Fig. 1. MiR-200c overexpression increases the radiosensitivity of breast cancer cells.

Fig. 1

a Relative expression of miR-200c in breast cancer cells and MCF-10A cells were detected using qRT-PCR. b Clonogenic survival assays of MDA-MB-231, BT549, SKBR3, T47D, BT474, and MCF-7 cells. c Relative expression of miR-200c in MDA-MB-231 and BT549 cells transduced with lentivirus encoding miR-200c or the empty vector. d Clonogenic survival assays of MDA-MB-231 and BT549 cells transduced with miR-200c. e Relative expression of miR-200c in MCF-7 and BT474 cells after transfection the miR-200c inhibitor. f Clonogenic survival assays of MCF-7 and BT474 cells transfected with the miR-200c inhibitor. g, h γ-H2AX foci formation analyzed by immunofluorescence in MDA-MB-231 cells transduced with miR-200c or empty vector, 24 h after 6 Gy IR. i Western blotting analysis of γ-H2AX expression in MDA-MB-231 cells transduced with miR-200c, at the indicated time points after 6 Gy IR. Data are presented as means ± SD, n = 3, *P < 0.05, **P < 0.01