Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 10;9:14595. doi: 10.1038/s41598-019-50768-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Flow cytometry-based time-course analysis of gexp02-tdTom and etramp10.3-tdTom expression during sexual development. (a) Schematic overview of the experiment design. Cultures were either maintained in the presence of choline, or with choline removed at the ring stage to induce sexual conversion. After reinvasion, the proportion of tdTom-positive parasites was determined by flow cytometry at several times (the times shown correspond to experiments with the gexp02-Tom lines, which were synchronized to a 5 h age window). ML10 was added at the trophozoite stage (generation 1, as indicated) to prevent schizont rupture and reinvasion. (b) Representative flow cytometry SYTO11 (stains DNA) – tdTomato plots for the NF54-gexp02-Tom and NF54-etramp10.3-Tom transgenic lines (- choline condition) at different times after invasion. Gates (rectangles) indicate SYTO11 and tdTomato double-positive parasites. (c) Quantification of sexual conversion rates at different times in the NF54-gexp02-Tom and E5-gexp02-Tom lines, based on detection of tdTomato fluorescence. Conversion rates (%) were determined as the gametocytemia relative to the initial rings parasitemia. Data are presented as the average and s.e.m. of three independent biological replicates. (d) Same as panel c, but for the NF54-etramp10.3-Tom and E5-etramp10.3-Tom lines. Data are presented as the average and s.e.m. of two independent biological replicates.