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. 2019 Oct 10;9:14532. doi: 10.1038/s41598-019-51074-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bland Altman plot comparing log-transformed sxtA and field-calibrated LSU assay derived estimates of A. catenella abundance. Points are means from triplicate qPCR assays of a single DNA extract. The sxtA assay significantly overestimated cell concentration in samples with fewer than 200 cells, a threshold that corresponds to about 5 cell equivalents or approximately 10³ sxtA copies per reaction. Were assays equivalent points would be tightly clustered about 0 across the full range of measured concentrations (x-axis).