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. 2019 Oct 10;10(10):772. doi: 10.1038/s41419-019-2004-4

Fig. 5. 1, 4-BQ induced autophagy and apoptosis by enhancing phosphorylation of Bcl-2 at Ser70 site and phosphorylation of beclin1 at Thr119 site.

Fig. 5

a–d The NQO1, Bcl-2, beclin1, and p62 were measured using ELISA assay after oxidative stress inhibitor(NAC) treatment. e–k Western blotting was used to analyze the level of phosphorylation of Bcl-2 at Ser70 site, phosphorylation of beclin1 at Thr119 site and autophagy- and apoptosis-associated proteins. Data are represented in the form of mean ± SD. *p < 0.05 compared to control group. #p < 0.05 compared to 20 μM 1,4-BQ-treated group. l The image of immunofluorescence was analyzed by Image J. Data are represented in the form of mean ± SD. *p < 0.05 compared to control group. #p < 0.05 compared to 20 μM 1,4-BQ-treated group