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. 2019 Oct 10;10(10):772. doi: 10.1038/s41419-019-2004-4

Fig. 7. 1, 4-BQ-induced apoptosis in turn enhanced autophagy.

Fig. 7

a After treating with apoptosis inhibitor (Z-VAD-FMK), the toxic effect of 1, 4-BQ on the expression of autophagy- and apoptosis-associated genes was investigated by qRT-PCR. b The Bcl-2, beclin1, and p62 were measured using ELISA assay after apoptosis inhibitor (Z-VAD-FMK) treatment. c Western blotting was used to analyze the level of proteins which were related to autophagy and apoptosis. d After treating with apoptosis inhibitor (Z-VAD-FMK), LC3B puncta was analyzed by image J. Data are represented in the form of mean ± SD. *p < 0.05 compared to control group. #p < 0.05 compared to 20 μM 1, 4-BQ-treated group