Fig. 1.
Expression pf ZIKV rE monomers and rE homodimers. a The soluble ZIKV rE-monomer (rEM, aa1–404) was expressed and purified from mammalian cells. The soluble stable ZIKV rE-homodimer (rED) was generated by an A264C substitution, resulting in a disulfide bridge in the E-domain II dimer interphase (yellow). b Purified rEM and rED were analyzed by SDS-PAGE and exhibited predicted molecular weights of ~49 kDa (monomer) and ~98 kDa (dimer). c Binding of ZIKV specific (A9E, G9E, and ZKA-230) and flavivirus cross-reactive (4G2, 1M7, C8, and C10) mAbs to ZIKV rEM and rED was analyzed. Binding of mAbs that have a quaternary footprint (Q) was only observed for rED. The DENV2 specific mAb 2D22 was used as a negative control. Results from n = 2 independent experiments are shown. Error bars represent standard deviation. Source data are provided as a Source Data file