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. 2019 Oct 10;9:14662. doi: 10.1038/s41598-019-49651-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Three-dimensional (3D) images of CAMAs (red) within a tentacle of the coral Acropora hyacinthus as visualized using FISH. (a) Section of tentacle showing localization of two types of CAMAs (10x magnification). (b) Single aggregation of bacteria in a large structure within the epithelium (40x magnification; composed of 92 z-stack images). (c) Multiple aggregations of bacteria in smaller structures within the gastrodermis (40x magnification; composed of 56 z-stack images). 3D rendering of CAMAs (d,e) reconstructed from 3D images in (b,c). Coral tissue and Symbiodiniaceae appear as blue and green structures, respectively.