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. 2019 Oct 4;6:100. doi: 10.3389/fmolb.2019.00100

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Copyright © 2019 Torosyan, Wiegand, Schledorn, Klose, Güntert, Böckmann and Meier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) 2D NCA spectrum (gray) and 2D plane of a 3D hCANH (cyan) spectrum at δ(¹H) = 7.6 ppm showing an example of the assignment transfer for 133Gly; (B) 2D NCA (gray) spectrum and 2D plane of a 3D hNCAH (blue) spectra at δ(¹H) = 3.7 ppm showing the example of the assignment transfer for 133Gly; (C) 2D plane of hCONH spectrum δ(¹H) = 7.6 ppm; (D) schematic representation of the assignment transfer for H^N and H^α atoms; (E) 2D hNH correlation spectrum of fully protonated Rpo4/7^* at 110 kHz MAS. The spectrum includes labels for the ¹⁵N-¹H peaks as predicted from the manually created shift list. On the right side of the figure 1D traces for ¹H are presented at the corresponding ¹⁵N frequencies. The ¹H linewidth characteristics of the full population of marked cross-peaks are summarized in the boxplot in the bottom right, indicating the maximum, 3rd quartile, mean, 1st quartile and minimum value of proton FWHM linewidth in Hz with a mean value of 160 ± 40 Hz. (F) 2D hCH correlation spectrum of fully protonated Rpo4/7^* at 110 kHz MAS with peaks labeled as in (E).