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. 2019 Oct 10;9:14588. doi: 10.1038/s41598-019-51088-x

Figure 3.

Figure 3

Socs3 Western blot bands at approximately 25 kDa (a). One band from each group has been shown. Mean intensity of Socs3 in the hindbrain (b), midbrain (c), left cortex (d) and right cortex (e). Mean ± SEM. Due to their large number, samples may have been run on different gels. A calibrator sample was repeated on each gel and results were calibrated to this prior to analysis. Total protein normalisation was performed with Ponceau S staining. Samples derive from the same experiment and blots were processed in parallel. Full blot images, raw data, total protein normalisation and calibrations are presented in Supplementary File S2. There was no HEM AM group at T1. n = pooled samples (individual mice): T1 AM: NON = 2(4), HOM = 2(4), T2 AM: NON = 2(4), HEM = 2(3), HOM = 2(3), T3 AM: NON = 2(3), HEM = 3(4), HOM = 5(8), T1 PM: NON = 3(5), HEM = 2(3) (left cortex = 1(2)), HOM = 2(4), T2 PM: NON = 5(10), HEM = 3(6), HOM = 3(6), T3 PM: NON = 4(7), HEM = 3(6), HOM = 1(2). *p < 0.05, differences between all other groups were nonsignificant.