Figure 4. Bioinformatic analysis of group I RFAP synthase genes. A. Multiple amino acid sequence alignment of Group I RFAP synthases using the alignment program GeneDoc [43]. Possible phosphate binding motifs are indicated by brackets. The protein abbreviations are as follows: Methanosarcina mazei, MM1592 and MM0322; Methanosarcina acetivorans, MA4006 and MA0339; Methanosarcina barkeri, MB2615; Methanopyrus kandleri, MK0558; Methanocaldococcus jannaschii, MJ1427; Archaeoglobus fulgidus, AF2089; Methanothermobacter thermautotrophicus, MTH0830; Sulfolobus tokodaii, ST1329; Sulfolobus sulfataricus, SSO0370. B. Multiple amino acid sequence alignment showing homology between the conserved region of RFAP synthase and a predicted pABA-binding region of dihydropteroate synthase (DHPS) from Staphylococcus aureus, Escherichia coli; Thermoanaerobacter tengcongensis, and Thermotoga maritima.