TABLE 1.
Primers used in this study given in 5′–3′ orientation.
| Name | Sequence 5′–3′ orientation |
| CHIKV E1 F | AAGCTCCGCGTCCTTTACCAAG |
| CHIKV E1 R | CCAAATTGTCCTGGTCTTCCT |
| Template Fluc F | GTGGTGTGCAGCGAGAATAG |
| Template Fluc R | CGCTCGTTGTAGATGTCGTTAG |
| GAPDH F | CCACATCGCTCAGACACCAT |
| GAPDH R | GGCAACAATATCCACTTTACCAGAGT |
| CHIKV nsP1 F | GGCAGTATCGTGAATTCGATGCGACACGGA GACGCCAACATT |
| CHIKV nsP1 R | AATAAATCATAAGTCTGCTCTCTGTCTACATGA |
| CHIKV nsP1 probe | [6FAM]TGCTTACACACAGACGT[TAM] |
| VCP-FL amplification F | TATAAGCTTATGGCTTCTGGAGCCGATTCAA |
| VCP-FL amplification R | TGCGGCCGCTTAGCCATACAGGTCATCATCATT |
| VCP-N amplification F | TATAAGCTTATGGCTTCTGGAGCCGATTCAA |
| VCP-N amplification R | TGCGGCCGCTTACAAGGACTCTTCCTCATCCTC |
| VCP-ND1 amplification F | TATAAGCTTATGGCTTCTGGAGCCGATTCAA |
| VCP-ND1 amplification R | TGCGGCCGCTTACTCTACCACGGTTTCCCGCA |
| D2 from FL F (SDM) | GTGCCACAGGTAACCTGG |
| D2 from FL R (SDM) | AAGCTTGTCATCGTCATCC |
Red indicates restriction sites. F, forward; R, reverse; SDM, site-directed mutagenesis.