Figure 3.

S100A11 promotes GBM EMT, migration and invasion. A, EMT‐associated proteins in indicated GBM cells were determined by Western blotting. GAPDH was used as the loading control. B and C, Transwell invasion assays of the invasive ability of the indicated GBM cells (*P < .05 and **P < .01). Results are mean ± s.e.m. from at least three independent assays. Scale bar, 100 μm. D and E, Wound‐healing assay of the migration ability of the indicated GBM cells (*P < .05 and **P < .01). Results are mean ± s.e.m. from at least 3 independent assays. Scale bar, 100 μm. F, 3D spheroid migration assay of the migration ability of the indicated GBM cells. Scale bar, 200 μm