Figure 4.

S100A11 facilitates the generation of GSCs. A, Western blot of S100A11 protein expression in neurospheres and adherent GBM cells. B, Representative immunofluorescence staining images of CD133 (red) and Nestin (green) in stem‐like U87 and U251 neurospheres. Scale bar, 20 μm. C, Western blot analysis of Sox2, Oct4 and Nanog in indicated GBM neurospheres. GAPDH was used as a loading control. D, Limiting dilution assay (LDA) was performed in indicated GBM spheres. E, Representative images of spheres formed in indicated GBM cells. Scale bar, 100 μm